BizTalk is notoriously hard to test. But I just came a cross a couple of interesting solutions to do unit testing for biztalk related logic and orchestration. BizMock : “BizMock is a framework for testing BizTalk solutions, using a Domain Driven Design
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Read the complete post at http://weblogs.asp.net/rosherove/archive/2010/01/12/unit-testing-biztalk-two-possible-frameworks.aspx